s high molecular weight chef dna lambda ladder (Bio-Rad)
Structured Review

S High Molecular Weight Chef Dna Lambda Ladder, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s high molecular weight chef dna lambda ladder/product/Bio-Rad
Average 94 stars, based on 192 article reviews
Images
1) Product Images from "Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study"
Article Title: Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study
Journal: Polish Journal of Environmental Studies
doi: 10.15244/pjoes/188905
Figure Legend Snippet: Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF DNA Lambda Ladder #1703707.
Techniques Used: Agarose Gel Electrophoresis, Staining, Software
Figure Legend Snippet: Fig. 2. Pulsed Field Gel Electrophoresis (PFGE) Genome Length Determination. a) PFGE for 13 phages out of 28 phages appear as single bands on PFGE which could be used to assess nucleic acid size of phages (1, 2, 3, 4, 8, 10, 11, 12, 13, 14, 15, 17) by comparing it to a ladder. PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.
Techniques Used: Pulsed-Field Gel, Electrophoresis, Agarose Gel Electrophoresis, Staining, Software
