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s high molecular weight chef dna lambda ladder  (Bio-Rad)


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    Bio-Rad s high molecular weight chef dna lambda ladder
    Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad <t>CHEF</t> DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF <t>DNA</t> <t>Lambda</t> Ladder #1703707.
    S High Molecular Weight Chef Dna Lambda Ladder, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s high molecular weight chef dna lambda ladder/product/Bio-Rad
    Average 94 stars, based on 192 article reviews
    s high molecular weight chef dna lambda ladder - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study"

    Article Title: Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study

    Journal: Polish Journal of Environmental Studies

    doi: 10.15244/pjoes/188905

    Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF DNA Lambda Ladder #1703707.
    Figure Legend Snippet: Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF DNA Lambda Ladder #1703707.

    Techniques Used: Agarose Gel Electrophoresis, Staining, Software

    Fig. 2. Pulsed Field Gel Electrophoresis (PFGE) Genome Length Determination. a) PFGE for 13 phages out of 28 phages appear as single bands on PFGE which could be used to assess nucleic acid size of phages (1, 2, 3, 4, 8, 10, 11, 12, 13, 14, 15, 17) by comparing it to a ladder. PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.
    Figure Legend Snippet: Fig. 2. Pulsed Field Gel Electrophoresis (PFGE) Genome Length Determination. a) PFGE for 13 phages out of 28 phages appear as single bands on PFGE which could be used to assess nucleic acid size of phages (1, 2, 3, 4, 8, 10, 11, 12, 13, 14, 15, 17) by comparing it to a ladder. PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.

    Techniques Used: Pulsed-Field Gel, Electrophoresis, Agarose Gel Electrophoresis, Staining, Software



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    Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad <t>CHEF</t> DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF <t>DNA</t> <t>Lambda</t> Ladder #1703707.
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    Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad <t>CHEF</t> DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF <t>DNA</t> <t>Lambda</t> Ladder #1703707.
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    Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF DNA Lambda Ladder #1703707.

    Journal: Polish Journal of Environmental Studies

    Article Title: Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study

    doi: 10.15244/pjoes/188905

    Figure Lengend Snippet: Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF DNA Lambda Ladder #1703707.

    Article Snippet: The gel was run using the Bio-Rad CHEF DRII system (BioRad, Richmond, CA, USA) in 0.5X TBE buffer for 18 h at 200 V with a switch time of 30 to 60 s. High molecular weight CHEF DNA Lambda Ladder #1703707 (BioRad) was used.

    Techniques: Agarose Gel Electrophoresis, Staining, Software

    Fig. 2. Pulsed Field Gel Electrophoresis (PFGE) Genome Length Determination. a) PFGE for 13 phages out of 28 phages appear as single bands on PFGE which could be used to assess nucleic acid size of phages (1, 2, 3, 4, 8, 10, 11, 12, 13, 14, 15, 17) by comparing it to a ladder. PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.

    Journal: Polish Journal of Environmental Studies

    Article Title: Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study

    doi: 10.15244/pjoes/188905

    Figure Lengend Snippet: Fig. 2. Pulsed Field Gel Electrophoresis (PFGE) Genome Length Determination. a) PFGE for 13 phages out of 28 phages appear as single bands on PFGE which could be used to assess nucleic acid size of phages (1, 2, 3, 4, 8, 10, 11, 12, 13, 14, 15, 17) by comparing it to a ladder. PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.

    Article Snippet: The gel was run using the Bio-Rad CHEF DRII system (BioRad, Richmond, CA, USA) in 0.5X TBE buffer for 18 h at 200 V with a switch time of 30 to 60 s. High molecular weight CHEF DNA Lambda Ladder #1703707 (BioRad) was used.

    Techniques: Pulsed-Field Gel, Electrophoresis, Agarose Gel Electrophoresis, Staining, Software

    Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF DNA Lambda Ladder #1703707.

    Journal: Polish Journal of Environmental Studies

    Article Title: Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study

    doi: 10.15244/pjoes/188905

    Figure Lengend Snippet: Fig. 1. Restriction enzyme gel and phylogenetic analysis dendrogram. a) Restriction digestion of AvrII enzyme pattern as separated by PFGE done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA) the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, running time = 9 hours, V= 6 V/cm, Temperature = 4ºC, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 3.0 software (BioRad); b) Phylogenetic analysis was performed and dendrogram was drawn by using BioNumerics software (Applied Maths NV). Phages were compared according to their genome digestion patterns. Bands were quite different for some phages while being similar for others, which considered to be similar phages due to having the same nucleic acid restriction patterns. c) BioRad CHEF DNA Lambda Ladder #1703707.

    Article Snippet: PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel DocTM XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.

    Techniques: Agarose Gel Electrophoresis, Staining, Software

    Fig. 2. Pulsed Field Gel Electrophoresis (PFGE) Genome Length Determination. a) PFGE for 13 phages out of 28 phages appear as single bands on PFGE which could be used to assess nucleic acid size of phages (1, 2, 3, 4, 8, 10, 11, 12, 13, 14, 15, 17) by comparing it to a ladder. PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.

    Journal: Polish Journal of Environmental Studies

    Article Title: Assessing the Biocontrol Potential of Some Isolated Bacteriophages Against Salmonella spp. in Food Preservation: A Preliminary Study

    doi: 10.15244/pjoes/188905

    Figure Lengend Snippet: Fig. 2. Pulsed Field Gel Electrophoresis (PFGE) Genome Length Determination. a) PFGE for 13 phages out of 28 phages appear as single bands on PFGE which could be used to assess nucleic acid size of phages (1, 2, 3, 4, 8, 10, 11, 12, 13, 14, 15, 17) by comparing it to a ladder. PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel Doc™ XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.

    Article Snippet: PFGE was done on BioRad CHEF DII system (Bio-Rad, Richmond, CA, USA), the following parameters (1% PFGE grade agarose gel, 0.5 X TBE running buffer, Running Time = 9 hours, V= 6 V/cm, Temp = 4°C, Switch Time = 30 to 60 seconds, GelStarTM nontoxic nucleic acid stain) and documentation was done on Gel DocTM XR+ UV Trans illumination and ImageLab 6.0 software (BioRad); b) BioRad CHEF DNA Lambda Ladder #1703707.

    Techniques: Pulsed-Field Gel, Electrophoresis, Agarose Gel Electrophoresis, Staining, Software

    FIGURE 1 High molecular weight structures identified by S1-PFGE. Sizes are indicated in Kb. M: molecular weight marker CHEF DNA Size Standard, 48.5-1,000 Kb, Lambda Ladder.

    Journal: Frontiers in cellular and infection microbiology

    Article Title: Plasmid content of carbapenem resistant Acinetobacter baumannii isolates belonging to five International Clones collected from hospitals of Alexandria, Egypt.

    doi: 10.3389/fcimb.2023.1332736

    Figure Lengend Snippet: FIGURE 1 High molecular weight structures identified by S1-PFGE. Sizes are indicated in Kb. M: molecular weight marker CHEF DNA Size Standard, 48.5-1,000 Kb, Lambda Ladder.

    Article Snippet: As molecular weight marker, CHEF DNA Size Standard, 48.5-1,000 Kb, Lambda Ladder (Bio-Rad) was used.

    Techniques: High Molecular Weight, Molecular Weight, Marker